Faststart high fidelity pcr system manual

Platinum SuperFi II DNA polymerase, a hot-start, high-fidelity PCR enzyme with x Taq fidelity, is ideal for applications requiring sequence accuracy during PCR. The included unique buffer is formulated for universal primer annealing at 60°C. The FastStart ™ High Fidelity PCR System — a new enzyme blend for hot start PCR — combines all the benefits of FastStart ™ Taq DNA Polymerase with fourfold higher accuracy and the abilty to amplify fragments up to 5 kb. FastStart ™ Taq DNA Polymerase is a thermostable, chemically modified form of recombinant Taq DNA Polymerase. The enzyme is inactive at +15 to +25°C during PCR setup, and then activated at +95°C during initial denaturation. This enzyme delivers superior results due to its unique enzyme design and optimized buffer system.

The FastStart™ High Fidelity PCR System is a blend of FastStart Taq DNA Polymerase and a thermostable proofreading protein, which is also chemically modified. This protein mediates proofreading activity, but carries no polymerase activity. Both proteins are inactive below +75 °C and are activated by heating to +95 °C for two minutes. FNR_PCR_RZ Uhr Seite 1 Probedruck C M Y CM MY CY CMY K www.doorway.ru PCR Applications Manual 3rd edition. The FastStart High Fidelity PCR System is the product of choice for multiplex PCR. Please see the online Application Note “Step-by-Step Protocol for Multiplex PCR with the FastStart High Fidelity PCR System and the PCR Optimization Kit” (Cat. No. 04 ).

The FastStart High Fidelity PCR System is a blend of FastStart Taq DNA Polymerase and a thermostable proofreading protein, which is also chemically modified. For fast high quality results, also use Roche′s PCR Optimization Kit.• Increase fidelity.• Achieve fourfold higher fidelity compared to Taq DNA Polymerase and. iProof is a high-fidelity DNA polymerase that offers extreme performance for all PCR applications. Incorporating an exciting new and patented technology, iProof.